Development of novel polymeric biomaterials, regeneration of tendon/ligament, protein delivery for orthopaedic tissue engineering.
The goal of our laboratory is to design polymeric biomaterials for specific orthopaedic applications, including regeneration of tendon/ligament, cartilage and bone. These synthetic and naturally-derived biomaterials are used in conjunction with other biochemical and mechanical stimuli to promote priming of stem cells to express a particular phenotype, as well as deliver biomolecules to promote healing of tissues that have degenerated due to chronic conditions, such as osteoarthritis or overuse injuries.
Cellular mechanics of hematologic processes and disease, microfluidics, microfabrication, BioMEMs, point-of-care diagnostics, pediatric medicine, hematology, oncology.
Our interdisciplinary laboratory, comprising clinicians, engineers, and biologists, is dedicated to applying and developing micro/nanotechnologies to study, diagnose, and treat blood disorders, cancer, and childhood diseases. This unique "basement to bench to bedside" approach to biomedical research is enabled by our lab’s dual locations at the Emory University School of Medicine and the Georgia Institute of Technology and our affiliations with the Children’s Healthcare of Atlanta hospitals.
Optical microscopy and in vivo imaging, RNA virus pathogenesis, HIV/SIV and hRSV, and detection, RNA regulation, therapeutics and vaccines.
Research in the Santangelo lab is primarily focused on three areas, native RNA regulation, RNA virus pathogenesis, and RNA therapeutics and vaccines, where the application and development of imaging technology is applied to all three areas. To address RNA regulation, localization and dynamics in the cellular milieu, we have developed single molecule sensitive approaches for imaging native RNAs and RNA dynamics in live cells, as well as the first assay to detect native RNA-protein interactions in situ. To date, the results of these methods have been applied to the cell biology of human respiratory syncytial virus infections and RNA regulation during tumorigenesis. These methods are also being used to interrogate and develop RNA-based therapeutics and vaccines. In addition we have been developing whole-body, PET/CT imaging tools for interrogating SIV infections within the macaque model. The purpose of this tool is to answer fundamental questions regarding the location of residual virus during treatment, in the hope of learning vital information that could be applied to approaches seeking to “cure” SIV or HIV.
It is our vision that one day mathematics and computation will be capable of reliably predicting, manipulating and optimizing biomedical systems for the advancement of medicine, drug development, biotechnology and productive and sustainable stewardship of the environment.
Pursuing this vision, the goal of our lab is to understand small biological systems in great detail. We work toward this goal by performing studies that target the fine-tuned synergism between genes, proteins and metabolites, and investigate the resultant, usually very effective functioning of healthy cells and organisms in comparison to those that are mutated or diseased. Milestones along the way are insights into the rationale for the intricate design and operation principles that govern biological systems.
The work in our lab is strictly mathematical and computational, but we collaborate with several superb experimental groups that provide us with data and appreciate our modeling efforts as tools for explanation and hypothesis generation.
Platt Lab Mission: To fuse engineering, cell biology, and physiology to understand how cells sense, respond, and remodel their immediate mechanical and biochemical environments for repair and regeneration in health and disease, then to translate that knowledge to clinics domestically and internationally to address global health disparities.
Biomechanics and mechanobiology of cell adhesion and signaling molecules of the immune system and the vascular systems:
Our interests lie in the adhesion and signaling molecules of the immune system as well as those involved in platelet adhesion and aggregation. We are primarily focused on early cell surface interaction kinetics and their primary signaling responses, as these are critical in determining how a cell will ultimately respond upon contact with another cell. The majority of our work ranges from single molecule interaction studies using atomic force microscopy, molecular dynamics simulations, or biomembrane force probe assays to single cell studies using micropipette adhesions assays, fluorescence imaging techniques, or real-time confocal microscopy. These assays focus on the mechanics and kinetics of receptor-ligand binding and their downstream signaling effects within cells. T cell receptors, selectins, integrins, and their respective ligands are some of the cell surface molecules currently under investigation in our lab. Understanding the initial interaction between molecules such as these and their subsequent early signaling processes is crucial to elucidating the response mechanisms of these physiological systems. Ultimately, our research strives to help better understand the mechanisms within these systems for possible medical applications in autoimmunity, allergy, transplant rejection, and thrombotic disorders.